Different from conventional reservoirs, fluid percolation in tight reservoirs does not conform to the classic Darcy's law, but has both dynamic starting pressure gradient and stress sensitive effect. The JBN method don't take these two factors into account when calculating relative permeability, which is bound to cause calculation errors. The seepage mechanism of tight gas reservoir was analyze, then a two phase seepage calculation method was built and verified considering the dynamic threshold pressure gradient and stress effect. The results show that when consider the dynamic threshold pressure gradient and stress effect, the seepage ability of gas and water phase both decrease obviously, and the decrease degree of water is bigger than the gas phase. The isotonic point of relative permeability decreases and the residue water saturation increases in rock sample. With the decline of pore pressure, the effect of dynamic threshold pressure gradient and stress becomes more and more significant. The new relative permeability curves calculated by the new method is more different with the curves calculated by the JBN method. The new method can describe the seepage of fluid in tight formation more accuracy.